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S set to -Wang et al. Virology Journal (2016) 13:Page 13 ofkCal/mol.

"Abbey Charette" (2020-05-25)


S set to -Wang et al. Virology Journal (2016) 13:Page 13 ofkCal/mol. Predicted targets were further filtered using more stringent criteria in which they had to contain either (1) a match between nucleotides 2? of the miRNA with the target sequence or (2) a match between nucleotides 2? and 13?6 of the miRNA with the target sequence (G:U base-pairing was tolerated). To reveal functions related to the putative target genes, Gene Ontology (GO) analysis was performed on predicted target gene candidates for conserved and novel miRNAs and differentially expressed miRNAs using three ontologies: molecular function, cellular components and biological process.2.3.4.5. 6. 7. 8.Additional filesAdditional file 1: Table S1. Conserved miRNAs from the nonviruliferous and viruliferous whitefly libraries (DOCX 21 kb) Additional file 2: Table S2. Induced miRNAs only in TYLCCNV viruliferous whiteflies (DOCX 14 kb) Additional file 3: Figure S1. GO classification of putative functions of targets of all conserved and novel miRNAs from the nonviruliferous and viruliferous whitefly libraries. The X axis shows subgroups of molecular functions from GO classification and the Y axis shows the number and the percent of the matched unigene sequences. (DOCX 362 kb) Additional file 4: Figure S2. GO classification of putative functions of targets of novel miRNA candidates from the nonviruliferous and viruliferous whitefly libraries. The X axis shows subgroups GS-441524 of molecular functions from GO classification and the Y axis shows the number and the percent of the matched unigene sequences. (DOCX 190 kb) Additional file 5: Table S3. Sequences of primers used in the study (DOCX 14 kb)9. 10. 11. 12.13.14.15.16. 17.Competing interests The authors declare that they have no competing interests. Authors' contributions XZ, SSL and XWW designed the whole study. BW, LW and MD performed the experiment. FC, XY, BW and ZZ analyzed the data. BW and XZ wrote the main manuscript text and prepared all figures. All authors reviewed the manuscript. All authors read and approved the final manuscript. Acknowledgements We thank Dr. Garry Sunter (University of Texas at San Antonio) for critical reading of PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26713783 the manuscript. This work was supported by National Natural Science Foundation of China (31390420 and U1136606). Author details 1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27832717 310058, People's Republic of China. 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, People's Republic of China. 3Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China. 4Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650223, People's Republic of China. Received: 6 November 2015 Accepted: 17 January18.19.20.21.22.23.24.25.26. References 1. Napoli C, Lemieux C, Jorgensen R. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in transgenic plant. Plant Cell. 1990;2:279?9.27.Cogoni C, Macino G. Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa. Proc Natl Acad Sci U S A. 1997;94:10233?. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and sp.