Morphological and functional changes in urinary bladder of multiparous female rats submitted to resistance exercise

Monise  Moreno; Fernanda Milani Magaldi; Cristiane Milani Magaldi; Fernando Luiz Affonso Fonseca; Eduardo Mazuco Cafarchio; Ricardo Aparecido Baptista Nucci; Monica Akemi  Sato; Laura Beatriz Mesiano  Maifrino

doi:10.23925/1984-4840.2021v23i3/4a7

Authors

Monise Moreno Universidade São Judas Tadeu. Laboratório de Estudos Morfológicos e Imuno-histoquímicos - São Paulo (SP), Brasil.
Fernanda Milani Magaldi Universidade São Judas Tadeu. Laboratório de Estudos Morfológicos e Imuno-histoquímicos - São Paulo (SP), Brasil. https://orcid.org/0000-0001-9412-4386
Cristiane Milani Magaldi Universidade São Judas Tadeu. Laboratório de Estudos Morfológicos e Imuno-histoquímicos - São Paulo (SP), Brasil. https://orcid.org/0009-0006-9016-6283
Fernando Luiz Affonso Fonseca Faculdade de Medicina do ABC. Laboratório de Análises Clínicas - Santo André (SP), Brasil. https://orcid.org/0000-0003-1223-1589
Eduardo Mazuco Cafarchio Faculdade de Medicina do ABC. Laboratório de Análises Clínicas - Santo André (SP), Brasil.
Ricardo Aparecido Baptista Nucci Universidade de São Paulo. Faculdade de Medicina. Departamento de Patologia - São Paulo (SP), Brasil https://orcid.org/0000-0001-5649-2145
Monica Akemi Sato Faculdade de Medicina do ABC. Laboratório de Análises Clínicas - Santo André (SP), Brasil. https://orcid.org/0000-0001-8627-363X
Laura Beatriz Mesiano Maifrino Faculdade de Medicina do ABC. Laboratório de Análises Clínicas - Santo André (SP), Brasil. https://orcid.org/0000-0002-9456-192X

DOI:

https://doi.org/10.23925/1984-4840.2021v23i3/4a7

Keywords:

urinary bladder, parity, exercise, pregnancy, incontinence urinary, rats

Abstract

Objective: investigate whether resistance exercise can induce morphological and functional alterations in urinary bladder (UB) in multiparous rats. Methods: we used 40 adults female Wistar rats of which 20 were nulliparous and 20 were multiparous submitted to different volumes of moderate resistance exercise (one, three or ten weeks). Animals were submitted to the functional evaluation of UB. At the end of the protocol, the UB was removed, weighed and stained with hematoxylin and eosin for structural evaluation, and picrosirius red for collagen fibers. Results: we observed that multiparity promoted increase in body mass, reduction in UB layers, decrease in volume densities of collagen fibers I and III. However, 10-weeks of training was able to reverse the negative effects of multiparity. Conclusion: the intervention of physical exercise in 10 weeks times seems to cause greater benefit in UB of multiparous animals by preventing morphofunctional changes that trigger lower urinary tract symptoms, such as urinary loss.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Soave I, Scarani S, Mallozzi M, Nobili F, Marci R, Caserta D. Pelvic floor muscle training for prevention and treatment of urinary incontinence during pregnancy and after childbirth and its effect on urinary system and supportive structures assessed by objective measurement techniques. Arch Gynecol Obstet. 2019;299(3):609-23. doi: 10.1007/s00404-018-5036-6.

Barbosa L, Boaviagem A, Moretti E, Lemos A. Multiparity, age and overweight/obesity as risk factors for urinary incontinence in pregnancy: a systematic review and meta-analysis. Int Urogynecol J. 2018;29(10):1413-27. doi: 10.1007/s00192-018-3656-9.

Moossdorff-Steinhauser HF, Berghmans B, Spaanderman ME, Bols EM. Prevalence, incidence and bothersomeness of urinary incontinence in pregnancy: a systematic review and meta-analysis. Int Urogynecol J. 2021;32(7):1633-52. doi: 10.1007/s00192-020-04636-3.

Chen L, Luo D, Chen X, Jin M, Yu X, Cai W. Development of predictive risk models of postpartum stress urinary incontinence for primiparous and multiparous women. Urol Int. 2020;104(9-10):824-32. doi: 10.1159/000508416.

Batmani S, Jalali R, Mohammadi M, Bokaee S. Prevalence and factors related to urinary incontinence in older adults women worldwide: a comprehensive systematic review and meta-analysis of observational studies. BMC Geriatr. 2021;21(1):212. doi: 10.1186/s12877-022-03111-6.

Magaldi FM, Moreno M, Magaldi CM, Cafarchio EM, Aronsson P, Sato MA, et al. Resistance exercise evokes changes on urinary bladder function and morphology in hypoestrogen rats. Front Physiol. 2020;10:1605. doi: 10.3389/fphys.2019.01605. eCollection 2019.

Araújo MPD, Oliveira ED, Zucchi EVM, Trevisani VFM, Girão MJBC, Sartori MGF. Relação entre incontinência urinária em mulheres atletas corredoras de longa distância e distúrbio alimentar. Rev Assoc Med Bras (1992). 2008;54(2):146-9. doi: 10.1590/S0104-42302008000200018

Fozzatti MCM, Palma P, Herrmann V, Dambros M. Impacto da reeducação postural global no tratamento da incontinência urinária de esforço feminina. Rev Assoc Med Bras (1992). 2008;54(1):17-22. https://doi.org/10.1590/S0104-42302008000100015

Bø K. Pelvic floor muscle training is effective in treatment of female stress urinary incontinence, but how does it work? Int Urogynecol J Pelvic Floor Dysfunct. 2004;15(2):76-84. doi: 10.1007/s00192-004-1125-0

Nucci RAB, Teodoro ACDS, Krause Neto W, Silva WDA, de Souza RR, Anaruma CA, et al. Effects of resistance training on liver structure and function of aged rats. Aging Male. 2018;21(1):60-4. doi: 10.1080/13685538.2017.1350157.

Cury JCS, Encinas, JA, Nucci RAB, Ornelas EM, de Lima NE, Braggion GF, et al. Effects of different diet intake and resistance training on left ventricle remodeling in ovariectomized rats. Comp Clin Path. 2019;28(6):1797-803. doi: 10.1007/s00580-019-03022-w.

Braggion GF, Ornelas EM, Cury JCS, de Sousa JP, Nucci RAB, Fonseca FLA, et al. Remodeling of the soleus muscle of ovariectomized old female rats submitted to resistance training and different diet intake. Acta Histochem. 2020;122(5):151570. doi: 10.1016/j.acthis.2020.151570.

Marcelino MCS, Magalhães WV, Fonseca FLA, Nucci RAB, Maifrino LBM. Effects of resistance training on kidney morphology of aged ovariectomized rats. Acta Histochem. 2020;122(7):151613. doi: 10.1016/j.acthis.2020.151613

Santana ESS, de Oliveira CA, Lima FIA, Nucci RAB, Fonseca FLA, Maifrino LBM. Effect of resistance training and diet intake on spleen structure of ovariectomized wistar rats. J Health Allied Sci NU. 2022;12(01):47-52. doi: 10.1055/s-0041-1732812.

Cafarchio EM, Da Silva LA, Auresco LC, Rodart IF, De Souza JS, Antonio BB, et al. Oxytocin reduces intravesical pressure in anesthetized female rats: Action on oxytocin receptors of the urinary bladder. Front Physiol. 2020;11:382. doi: 10.3389/fphys.2020.00382

Mandarim-de-Lacerda CA. Stereological tools in biomedical research. An Acad Bras Ciênc. 2003;75(4):469-86. doi: 10.1590/S0001-37652003000400006

Rodriguez CP, Ogunmoroti O, Quispe R, Osibogun OI, Ndumele CE, Tcheugui JE, et al. The association between multiparity and adipokine levels: the multi-ethnic study of atherosclerosis. J Womens Health (Larchmt). 2022;31(5):741-9. doi: 10.1089/jwh.2021.0091.

Moreira SFDS, Girão MJBC, Sartori MGF, Baracat EC, Lima GRD. Mobilidade do colo vesical e avaliação funcional do assoalho pélvico em mulheres continentes e com incontinência urinária de esforço, consoante o estado hormonal. Rev Bras Ginecol Obstet. 2002;24:365-70. doi: 10.1590/S0100-72032002000600002.

Tawfik HE, Cena J, Schulz R, Kaufman S. Role of oxidative stress in multiparity-induced endothelial dysfunction. Am J Physiol Heart Circ Physiol. 2008;295(4):H1736-42. doi: 10.1152/ajpheart.87.2008.

Galea LA, Leuner B, Slattery DA. Hippocampal plasticity during the peripartum period: influence of sex steroids, stress and ageing. J Neuroendocrinol. 2014;26(10):641-8. doi: 10.1111/jne.12177.

Perucchini D, DeLancey JO, Ashton-Miller JA, Peschers U, Kataria T. Age effects on urethral striated muscle I. Changes in number and diameter of striated muscle fibers in the ventral urethra. Am J Obstet Gynecol. 2002;186(3):351-5. doi: 10.1067/mob.2002.121089.

Damaser MS, Broxton-King C, Ferguson C, Kim FJ, Kerns JM. Functional and neuroanatomical effects of vaginal distention and pudendal nerve crush in the female rat. J Urol. 2003;170(3):1027-31. doi: 10.1097/01.ju.0000079492.09716.43.

Chess-Williams R. Muscarinic receptors of the urinary bladder: detrusor, urothelial and prejunctional. Auton Autacoid Pharmacol. 2002;22(3):133-45. doi: 10.1046/j.1474-8673.2002.00258.x.

Chapple CR, Yamanishi T, Chess-Williams R. Muscarinic receptor subtypes and management of the overactive bladder. Urology. 2002;60(5):82-88. doi: 10.1016/s0090-4295(02)01803-4.

Fowler CJ, Griffiths D, De Groat WC. The neural control of micturition. Nat Rev Neurosci. 2008;9(6):453-66. doi: 10.1038/nrn2401.

Rocha MA, Sartori MGF, Simoes, MJ, Herrmann V, Baracat EC, de Lima GR, et al. The impact of pregnancy and childbirth in the urethra of female rats. Int Urogynecol J Pelvic Floor Dysfunct. 2007;18(6):645-51. doi: 10.1007/s00192-006-0221-8.

Drake MJ, Hedlund P, Mills IW, McCoy R, McMurray G, Gardner BP, et al. Structural and functional denervation of human detrusor after spinal cord injury. Lab Invest. 2000;80(10):1491-9. doi: 10.1038/labinvest.3780158.

Genadry R. A urogynecologist’s view of the pelvic floor effects of vaginal delivery/cesarean section for the urologist. Curr Urol Rep. 2006;7(5):376-83. doi: 10.1007/s11934-006-0007-z.

Liu X, Zhao Y, Pawlyk B, Damaser M, Li T. Failure of elastic fiber homeostasis leads to pelvic floor disorders. Am J Pathol. 2006;168(2):519-28. doi: 10.2353/ajpath.2006.050399.