Frequência de micronucleação e outras alterações nucleares em células da mucosa bucal de pacientes anêmicos

Bianca Caroline Alvim Tomaz; Raissa Negrelli da Silva Ferri; Júlio Boschini Filho

doi:10.5327/Z1984-4840201626811

Authors

Bianca Caroline Alvim Tomaz Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo
Raissa Negrelli da Silva Ferri Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo
Júlio Boschini Filho Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo

DOI:

https://doi.org/10.5327/Z1984-4840201626811

Keywords:

anemia, genotoxicity, micronucleus tests

Abstract

Introduction: The Micronucleus Cytogenetic Test is a biomarker that provides information to assess chromosomal damage which arises from genotoxic actions. The micronucleus frequency can be measured at situations of substances and environmental exposure with high genotoxic power. Many anemias have their genesis in nutritional deficiencies and have the consequence of an increment in DNA lesions, altogether with high oxidative stress. Folic acid and B12 vitamin determines more chromosomal instability, such cofactors deficiency or oxidative lesions in genetic material, with implications in DNA repair system. Objectives: Considering the correlation of the anemias and damage to genetic material, the present study aims to evaluate the micronucleation rate and other nuclei alterations in oral mucosal cells of anemic adults. Methods: This study was conducted with 60 patients, 30 of them were anemic and 30 were in the control group. The samples were collected and processed according to a pre-established protocol and thereafter slides were microscopically evaluated for proliferation, genotoxicity, cytotoxicity and cell death criteria. For statistical analysis, Shapiro-Wilk test, Student’s t-test, Levene test and Pearson correlation test were used to compare the variables. Results: The micronucleation rate and the others alterations were higher in anemic, while the control group showed a higher cellular proliferative capacity. Also, way of life influenced the cellular criteria assessed. Conclusions: The high frequency of micronuclei in anemia was attributed to oxidative stress, with malnutrition or other predisposing conditions such as genetic etiology of anemia or the presence of chronic disease.

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Author Biographies

Bianca Caroline Alvim Tomaz, Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo

Acadêmica de Medicina da FCMS/ PUC-SP

Raissa Negrelli da Silva Ferri, Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo

Acadêmica de Medicina da FCMS/ PUC-SP

Júlio Boschini Filho, Faculdade de Ciências Médicas da Pontifícia Universidade Católica de São Paulo

Professor Titular do Departamento de Morfologia e Patologia

Área de Biologia Molecular

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