Efeito do estresse sobre o metabolismo glicídico de camundongos tornados obesos

Gabriella Fernandes Gozoli; Paula Regina Zamuner Zeca; Bruna Calzzetta; Mário Luís Ribeiro Cesaretti

doi:10.23925/1984-4840.2017v19i3a5

Authors

Gabriella Fernandes Gozoli
Paula Regina Zamuner Zeca PUC-SP/FCMS
Bruna Calzzetta PUC-SP/FCMS
Mário Luís Ribeiro Cesaretti

DOI:

https://doi.org/10.23925/1984-4840.2017v19i3a5

Keywords:

sleep, REM, obesity, glucose metabolism disorders, mice

Abstract

Introduction: Sleep disorders, alone or in association with a high-calorie diet, may determine metabolic changes in the autonomic nervous system and in the hypothalamic-pituitary-adrenal axis, which may increase glycemia and produce glucose intolerance. The state of glucose intolerance usually precedes the onset of type 2 diabetes. Objective: This study aims to evaluate the effects of the association of paradoxical sleep deprivation and hypercaloric diet on the eating behavior and glucose tolerance of mice. Methods: Swiss mice were distributed into six groups: (1) control; (2) hypercaloric diet; (3) paradoxical sleep deprivation; (4) paradoxical sleep deprivation + hypercaloric diet; (5) monossodium glutamate (PSP); and (6) paradoxical sleep deprivation + monossodium glutamate. During the eight-week follow-up, food consumption was assessed and mice were weighed periodically. Sleep deprivation was performed weekly. After eight weeks, the oral glucose tolerance (TTGO) and insulin sensitivity test (TTI) were performed. At the end, heart and epididymal fat were weighed. Results: There was a significant increase in body weight in obese mice, which was associated with increased visceral fat content. All animals, obese and PSP, showed worsening in the parameters that measured the glucose metabolism, either on TTGO and TTI. There was no change in cardiac weight. Conclusion: The administration of a hypercaloric diet and paradoxical sleep deprivation determine glucose intolerance and insulin resistance, which in this work was not synergistic.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biography

Gabriella Fernandes Gozoli

PUC-SP/FCMS

References

Spiegel K, Tasali E, Penev P, Van Cauter E. Brief communication: sleep curtailment in healthy young men is associated with decreased leptin levels, elevated ghrelin levels, and increased hunger and appetite. Ann Intern Med. 2004;141(11):846-50.

Boergers J, Gable CJ, Owens JA. Later school start time is associated with improved sleep and daytime functioning in adolescents. J Dev Behav Pediatr. 2014;35(1):11-7.

Scott LD, Hwang W-T, Rogers AE, Nysse T, Dean GE, Dinges DF. The relationship between nurse work schedules, sleep duration, and drowsy driving. Sleep. 2007;30(12):1801-7.

Ayas NT, White DP, Manson JE, Stampfer MJ, Speizer FE, Malhotra A, et al. A prospective study of sleep duration and coronary heart disease in women. Arch Intern Med. 2003;163(2):205-9.

Reutrakul S, Van Cauter E. Interactions between sleep, circadian function, and glucose metabolism: implications for risk and severity of diabetes. Ann N York Acad Sci. 2014;1311:151-73.

Misra A, Khurana L. Obesity and the metabolic syndrome in developing countries. J Clin Endocrinol Metab. 2008;93(11 Suppl. 1):S9-30.

Muñoz-Pareja M, Loch MR, Santos HG, Bortoletto MS, Durán González A, Andrade SM. Factores asociados a mala calidad de sueño en población brasilera a partir de los 40 años de edad: estudio VIGICARDIO. Gac Sanit. 2016;30(6):444-50.

Pillar G, Shehadeh N. Abdominal fat and sleep apnea: the chicken or the egg? Diabetes Care. 2008;31(Suppl. 2):S303-9.

Morgenstern M, Wang J, Beatty N, Batemarco T, Sica AL, Greenberg H. Obstructive sleep apnea: an unexpected cause of insulin resistance and diabetes. Endocrinol Metab Clin North Am. 2014;43(1):187-204.

Cesaretti MLR. Modelos experimentais de resistência à insulina e obesidade: lições aprendidas. Endocrinol Metab. 2006;50:190-7.

Colditz GA, Philpott SE, Hankinson SE. The impact of the nurses’ health study on population health: prevention, translation, and control. Am J Public Health. 2016;106(9):1540-5.

Nilsson PM, Rööst M, Engström G, Hedblad B, Berglund G. Incidence of diabetes in middle-aged men is related to sleep disturbances. Diabetes Care. 2004;27(10):2464-9.

Meisinger C, Heier M, Loewel H, MONICA/KORA Augsburg Cohort Study. Sleep disturbance as a predictor of type 2 diabetes mellitus in men and women from the general population. Diabetologia. 2005;48(2):235-41.

Knutson KL. Impact of sleep and sleep loss on glucose homeostasis and appetite regulation. Sleep Med Clin. 2007;2(2):187-97.

González-Ortiz M, Martínez-Abundis E. Impact of sleep deprivation on insulin secretion, insulin sensitivity, and other hormonal regulations. Metab Syndr Relat Dis. 2005;3(1):3-7.

Valensi P, Pariès J, Attali JR, French Group for Research and Study of Diabetic Neuropathy. Cardiac autonomic neuropathy in diabetic patients: influence of diabetes duration, obesity, and microangiopathic complications – the French multicenter study. Metab Clin Exp. 2003;52(7):815-20.

Gudbjörnsdottir S, Friberg P, Elam M, Attvall S, Lönnroth P, Wallin BG. The effect of metformin and insulin on sympathetic nerve activity, norepinephrine spillover and blood pressure in obese, insulin resistant, normoglycemic, hypertensive men. Blood Press. 1994;3(6):394-403.

Spiegel K, Leproult R, Van Cauter E. Impact of sleep debt on metabolic and endocrine function. Lancet. 1999;354(9188):1435-9.

Woods SC, D’Alessio DA. Central control of body weight and appetite. J Clin Endocrinol Metab. 2008;93(11 Suppl. 1):S37-50.

Koban M, Swinson KL. Chronic REM-sleep deprivation of rats elevates metabolic rate and increases UCP1 gene expression in brown adipose tissue. Am J Physiol Endocrinol Metab. 2005;289(1):E68-74.

van Dijk G, van Heijningen S, Reijne AC, Nyakas C, van der Zee EA, Eisel ULM. Integrative neurobiology of metabolic diseases, neuroinflammation, and neurodegeneration. Front Neurosci. 2015;9:173.

Ferreira CBND, Cesaretti MLR, Ginoza M, Kohlmann O. Metformin effects upon blood pressure and glucose metabolism of monossodium glutamate induced-obese spontaneously hypertensive rats. Arq Bras Endocrinol Metab. 2009;53:409-15.

Voltera AF, Cesaretti MLR, Ginoza M, Kohlmann O. Effects of neuroendocrine obesity induction on systemic hemodynamics and left ventricular function of normotensive rats. Arq Bras Endocrinol Metab. 2008;52:47-54.

Briançon-Marjollet A, Weiszenstein M, Henri M, Thomas A, Godin-Ribuot D, Polak J. The impact of sleep disorders on glucose metabolism: endocrine and molecular mechanisms. Diabetol Metab Syndr. 2015;7:25.

Van Cauter E, Spiegel K, Tasali E, Leproult R. Metabolic consequences of sleep and sleep loss. Sleep Med. 2008;9(Suppl. 1):S23-8.

Verma S, Hussain ME. Obesity and diabetes: an update. Diabetes Metab Syndr. 2016;11(1):73-9.

Crönlein T. Insomnia and obesity. Curr Opin Psychiatry. 2016;29(6):409-12.

Wang Y, Carreras A, Lee S, Hakim F, Zhang SX, Nair D, et al. Chronic sleep fragmentation promotes obesity in young adult mice. Obesity. 2014;22(3):758-62.

Muntzel MS, Al-Naimi OAS, Barclay A, Ajasin D. Cafeteria diet increases fat mass and chronically elevates lumbar sympathetic nerve activity in rats. Hypertension. 2012;60(6):1498-502.

Zeeni N, Dagher-Hamalian C, Dimassi H, Faour WH. Cafeteria diet-fed mice is a pertinent model of obesityinduced organ damage: a potential role of inflammation. Inflamm Res. 2015;64(7):501-12.